Sweetpotato Germplasm and Genome Resource Database

CRISPR / SPACER SELECTION

Cas9 Design

Find genome-aware SpCas9 sgRNAs by gene or interval, then inspect every exact genomic hit before choosing a target.

5′ protospacer SpCas9 · NGG
20 nt guide edit window 3–5 PAM

CANDIDATES

Guide selection

Run a query to inspect candidate sgRNAs.

Start with a biological target

Choose one of six genome indexes, then search a gene, a genomic interval or an existing 20 nt guide.

  1. 1Pick the matching assembly
  2. 2Limit candidates by annotation and GC
  3. 3Prefer fewer exact genomic hits